Transient particle tracking microrheology of plasma coagulation via the intrinsic pathway

Abstract The maintenance of hemostasis to ensure vascular integrity is dependent upon the rapid conversion zymogen species coagulation cascade their enzymatically active forms. This process culminates in generation serine protease thrombin and polymerization fibrin prevent leak at sites endothelial cell injury or loss cellular junctions. Thrombin can be initiated by extrinsic pathway through exposure blood tissue factor damage, alternatively (F) XII activated foreign surfaces with negative charges, such as glass, contact activation pathway. Here, we used transient particle tracking microrheology investigate mechanical properties response downstream both pathways. We found that structural heterogeneity formation was on reaction kinetics generation. Pharmacological inhibition FXII activity prolonged time form increased degree fibrin, resulting clots reduced properties. Taken together, this study demonstrates a dependency physical biology coagulation.